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Related Experiment Videos

Peroxynitrite decreases rabbit tissue factor activity in vitro.

Vance G Nielsen1, John P Crow

  • 1Department of Anesthesiology, The Center for Free Radical Biology, The University of Alabama at Birmingham, Birmingham, AL 35249-6810, USA. vance.nielsen@cc.uab.edu

Anesthesia and Analgesia
|February 26, 2004
PubMed
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Peroxynitrite (OONO(-)) significantly reduces rabbit tissue factor (TF) activity, a key initiator of blood clotting. This finding may explain coagulopathy seen in hepatoenteric ischemia-reperfusion injuries.

Area of Science:

  • Biochemistry
  • Physiology
  • Pathology

Background:

  • Tissue factor (TF) is crucial for initiating physiological coagulation.
  • Hepatoenteric ischemia-reperfusion injury is linked to coagulopathy and peroxynitrite (OONO(-)) formation.
  • Circulating TF activity decreases in rabbits following hepatoenteric ischemia-reperfusion.

Purpose of the Study:

  • To investigate the effect of peroxynitrite (OONO(-)) on rabbit tissue factor (TF) activity in vitro.
  • To test the hypothesis that OONO(-) exposure decreases rabbit TF activity.

Main Methods:

  • Rabbit brain TF was incubated with varying concentrations of 3-morpholinosydnonimine (SIN-1) to generate OONO(-).
  • Recombinant human superoxide dismutase (hSOD1) was used to neutralize superoxide.

Related Experiment Videos

  • TF activity was measured using thrombelastography by assessing clot formation kinetics in human plasma.

    • Main Results:

    • Exposure of rabbit TF to SIN-1 (generating OONO(-)) resulted in a significant 48% decrease in TF activity.
    • No significant differences in TF activity were observed between control groups (no SIN-1, hSOD1 only).

    Conclusions:

    • Peroxynitrite (OONO(-)) inhibits rabbit tissue factor (TF) activity in vitro.
    • Further research is warranted to elucidate the role of OONO(-) in coagulopathies associated with hepatoenteric ischemia-reperfusion.