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Phylogenetic relationships in Bupleurum (apiaceae) based on nuclear ribosomal DNA its sequence data.

Susana S Neves1, Mark F Watson

  • 1Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, UK. sneves@itqb.unl.pt

Annals of Botany
|February 26, 2004
PubMed
Summary

Phylogenetic analysis of Bupleurum using DNA sequencing reveals two major clades, challenging existing classifications. This study clarifies the evolutionary relationships and biogeography of Bupleurum species.

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Area of Science:

  • Botany
  • Molecular Systematics
  • Evolutionary Biology

Background:

  • The genus Bupleurum is recognized, but its infrageneric classification remains controversial.
  • Previous classifications have not been validated with molecular sequence data.

Purpose of the Study:

  • To investigate the phylogenetic relationships within the genus Bupleurum using DNA sequence data.
  • To provide molecular insights into the controversial infrageneric classification of Bupleurum.

Main Methods:

  • Comparative sequencing of the ITS region of the 18-26S nuclear ribosomal DNA repeat for 32 Bupleurum species.
  • Phylogenetic analyses using maximum parsimony, maximum likelihood, and neighbour-joining methods.

Main Results:

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  • Phylogenetic analyses consistently show Bupleurum as monophyletic, divided into two major clades.
  • One clade comprises species with pinnate-reticulate leaf venation and B. rigidum; the other includes species with parallel-veined leaves.
  • The 5.8S coding sequence analysis supports this two-clade division.

    • Conclusions:

    • The molecular phylogeny contradicts previous classifications of the Bupleurum genus.
    • Molecular data suggest B. salicifolium is a neoendemic, with small sequence divergence from mainland relatives.
    • Endemic Macaronesian and North African taxa form a well-supported clade, indicating recent radiation.