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[Comparative study on HAV and Poliovirus 2 inactivation cinetics with peracetic acid]

G Sansebastiano1, R Zoni, L Bigliardi

  • 1Università di Parma, Dipartimento di Sanità Pubblica, Sezione di Igiene. giulianoezio.sansebastiano@iperuniv.cce.it

Igiene E Sanita Pubblica
|February 26, 2004
PubMed

Insights

Peracetic acid effectively inactivates Hepatitis A virus (HAV) and Poliovirus 2. Higher concentrations and acidic pH accelerate viral inactivation, with HAV showing greater resistance.

Area of Science:

  • Virology
  • Disinfection Science
  • Public Health

Background:

  • Hepatitis A virus (HAV) and Poliovirus are significant public health concerns.
  • Effective disinfection methods are crucial for preventing viral transmission.
  • Peracetic acid is a widely used disinfectant with known antimicrobial properties.

Purpose of the Study:

  • To evaluate the efficacy of peracetic acid in inactivating Hepatitis A virus (HAV) and Poliovirus 2.
  • To determine the influence of peracetic acid concentration and pH on viral inactivation rates.
  • To compare the susceptibility of HAV and Poliovirus 2 to peracetic acid disinfection.

Main Methods:

  • Inactivation tests were performed using three peracetic acid concentrations (160, 320, 640 mg/l) at varying pH and 20°C.
  • HAV (HM-175 strain) was titrated using plaque-forming units (PFU).
  • Poliovirus 2 was titrated using 50% tissue culture infectious dose (TCID50).
  • Viral reduction was monitored over one hour with 10-15 minute intervals.

Main Results:

  • Hepatitis A virus inactivation showed a linear trend with contact time at higher peracetic acid concentrations, irrespective of pH.
  • Complete inactivation of HAV was achieved within 30-60 minutes at 640 mg/l.
  • Poliovirus 2 inactivation exhibited a linear trend with contact time across all concentrations, with faster inactivation at acidic pH.
  • Complete inactivation of Poliovirus 2 occurred within 30 minutes at 640 mg/l and acidic pH.
  • HAV demonstrated higher resistance to peracetic acid compared to Poliovirus 2 and Poliovirus 1.

Conclusions:

  • Peracetic acid is an effective disinfectant against Hepatitis A virus and Poliovirus 2.
  • Disinfection efficacy is concentration-dependent and influenced by pH, particularly for Poliovirus 2.
  • Hepatitis A virus requires longer contact times or higher concentrations for complete inactivation compared to Poliovirus 2.

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