Determination of sulphite using an immobilized enzyme with flow injection chemiluminescence detection

A flow injection method is reported for the determination of sulphite-based on chemiluminescent detection. Hydrogen peroxide is produced from sulphite using on-line covalently bound immobilized sulphite oxidase packed in a mini-column, which was mixed downstream and detected via cobalt(II)-catalysed chemiluminescent oxidation of luminol. The limit of detection (2 x standard deviation of the blank) was 1 x 10(-3) mmol/L with sample throughput 60 h(-1). The calibration data was linear over the range of 0.2-1.0 mmol/L with relative standard deviation (n = 4) in the range 0.9-2.0%.