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Updated: Aug 26, 2026

Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
Published on: September 13, 2019
[Study on microtubulin of human rhabdomyosarcoma]
Lu Ye1, Hong-ying Zhang, Hong Bu
1Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To detect the expression of microtubules in human rhabdomyosarcoma.
Methods:
Expression of microtubulin-beta in 70 tissue slides from human rhabdomyosarcoma was detected by immunohistochemistry. Immunofluorescent staining was used to examine microtubules in PLA-802, A673 and RD under laser scanning confocal microscope.
Results:
Expression of microtubulin-beta in human rhabdomyosarcoma tissue was widely decreased. The low expression rate of microtubulin-beta in histological grade-I tumor was 52.38%, and that in histological grade-III tumor 85% (P < 0.05). The low expression rate of microtubulin-beta in 32 cases with metastasis and recurrence was 87.5%, and that in 38 cases without metastasis and recurrence 55.26%. Microtubules in the three cell lines showed different degrees of maldevelopment. The most severe degree was observed in PLA-802, and the less severe degree in RD (P < 0.05).
Conclusion:
The microtubulin shows extensive low expression in human rhabdomyosarcoma. The decrease and hypogenesis of microtubules in human rhabdomyosarcoma have relationship with tumor's histological grade, malignant degree, metastasis, and recurrence.
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