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Using in vitro human tissues to predict pharmacokinetic properties.
Norman D Huebert1, Malini Dasgupta, Yanmin Chen
13-Dimensional Pharmaceuticals Inc, 665 Stockton Drive, Exton, PA 19341, USA. nhuebert@prdus.jnj.com
Current Opinion in Drug Discovery & Development
|February 26, 2004
Summary
In vitro assays using human materials help predict drug candidate success early in development. These models improve understanding of drug absorption, distribution, metabolism, and excretion (ADME) for better drug design.
Area of Science:
- Pharmacology
- Drug Discovery
- Biotechnology
Background:
- Drug discovery faces pressure to predict candidate success early.
- Species-specific biotransformations and transport impact drug bioavailability.
- In vitro assays using human tissues, cells, or fluids are crucial.
Purpose of the Study:
- To highlight the role of in vitro assays in predicting drug candidate success.
- To explain how these assays aid chemists and biologists in decision-making.
- To demonstrate the application of these models in understanding in vivo pharmacokinetic outcomes.
Main Methods:
- Development of in vitro assays utilizing human tissues, cells, and fluids.
- Application of these models for screening and ranking drug candidates.
- Analysis of absorption, distribution, metabolism, and excretion (ADME) liabilities.
Main Results:
- In vitro assays effectively indicate potential liabilities in drug scaffolds.
- These models aid in understanding mechanisms of in vivo pharmacokinetic outcomes.
- Development of structure-ADME property relationships is facilitated.
Conclusions:
- In vitro assays are vital for early prediction of drug candidate success.
- These models contribute to understanding drug bioavailability and pharmacokinetics.
- Future development aims for in silico models for predictive drug assessment.