Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pathways to acute humoral rejection.

Soheyla Saadi1, Takao Takahashi, Robert A Holzknecht

  • 1Department of Surgery, Mayo Clinic, Rochester, Minnesota 55905, USA.

The American Journal of Pathology
|February 26, 2004
PubMed
Summary

Interleukin-1 (IL-1) plays a key role in acute humoral rejection, a major hurdle in organ transplantation and xenotransplantation. Blocking IL-1 signaling effectively prevented early inflammatory gene expression and tissue damage in a rat heart transplant model.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TNFRSF13B Common Variants Enhance Antibody-Dependent Complement Activation and Susceptibility to Acute Respiratory Distress Syndrome Following Respiratory Viral Infection.

medRxiv : the preprint server for health sciences·2026
Same author

Xenotransplantation: From research to trial to practice.

Human immunology·2025
Same author

Mineralocorticoid receptor phase separation modulates cardiac preservation.

Nature cardiovascular research·2025
Same author

Piwi-interacting RNAs (piRNAs), potential new liquid biopsy in the immune surveillance of heart transplant recipients.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2025
Same author

Complement C3d enables cell-mediated immunity capable of distinguishing spontaneously transformed from nontransformed cells.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Complement C3d enables protective immunity capable of distinguishing spontaneously transformed from non-transformed cells.

bioRxiv : the preprint server for biology·2024

Area of Science:

  • Transplantation immunology
  • Inflammation research
  • Xenotransplantation

Background:

  • Acute humoral rejection (AHR) is a significant challenge in organ transplantation, particularly in xenotransplantation.
  • While antibodies and complement are implicated, other factors like ischemia and immune cells may also contribute to AHR.
  • Understanding the pathways initiating AHR is crucial for developing prevention strategies.

Purpose of the Study:

  • To investigate the role of interleukin-1 (IL-1) in the early pathogenesis of acute vascular rejection.
  • To determine if IL-1 acts as a central mediator in the inflammatory cascade during AHR.

Main Methods:

  • A heterotopic guinea pig-to-rat heart transplant model was utilized.
  • Rats were treated with cobra venom factor (CVF) to induce severe vascular rejection.

Related Experiment Videos

  • Interleukin-1 receptor antagonist (IL-1ra) was administered to block IL-1 signaling.

    • Main Results:

    • Early stages of acute vascular rejection were characterized by the expression of inflammatory genes.
    • IL-1 availability appeared to control the expression of these inflammatory genes.
    • IL-1ra treatment prevented gene expression and subsequent tissue damage, including coagulation and inflammatory cell infiltration.

    Conclusions:

    • Interleukin-1 (IL-1) plays a critical role in initiating acute humoral rejection.
    • Targeting IL-1 signaling presents a potential therapeutic strategy to prevent early graft injury in AHR.