[The detection and clinical significance of three enzymes in gingival crevicular fluid]

To determine the possibility of type collagenase(COL- ), aspartate aminotransferase(AST), elastase in the supernatant(EA-s) in gingival crevicular fluid(GCF) as the diagnostic parameters for early periodontal disease through determining their levels in GCF.

GCF was sampled with filter strips by entrance-pocket method from ninety patients. The levels of COL-, AST and EA-s were determined separately by ELISA, Roche automatic biochemistry analyzer and the substrate Meosuc-ala-ala-pro-val-NA.

The levels of COL-, AST, EA-s were significantly different among periodontitis, gingivitis and healthy group(P<0.001). The GCF volume, probing depth, attachment loss and sulcus bleeding index were positively related to the levels of enzymes. There were also positive relationships among these three enzymes.

COL-, AST, EA-s could be helpful in early diagnosis for periodontal disease.