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Mutations in TAP genes are common in cervical carcinomas
1Centre for Immunology and Cancer Research, University of Queensland, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia.
Gynecologic Oncology
|February 27, 2004
Summary
Mutations in Human Leukocyte Antigen (HLA) class I and transporter of antigen processing (TAP) genes were found in cervical cancers. TAP gene alterations may play a role in cervical cancer development from precursor lesions.
Area of Science:
- Immunogenetics
- Oncology
- Molecular Biology
Background:
- Cervical cancer is a significant global health concern.
- Human Leukocyte Antigen (MHC class I) and transporter of antigen processing (TAP) genes are crucial for immune surveillance.
- Alterations in these genes may contribute to cancer development.
Purpose of the Study:
- To investigate mutations and deletions in MHC class I and TAP genes in squamous cervical cancers.
- To compare gene alterations in cervical carcinomas with precursor lesions (CIN2-3).
Main Methods:
- Polymerase chain reaction (PCR) based protocols, including sequence-specific primer PCR (SSP-PCR), were employed.
- HLA class I and TAP genes were analyzed in cervical tumor DNA.
- DNA from corresponding blood samples served as controls.
Main Results:
- Mutations in HLA-A genes were detected in 26% of cervical carcinomas.
- Mutations in TAP genes were found in 52% of cervical carcinomas.
- TAP gene mutations were significantly less frequent in CIN2-3 lesions compared to carcinomas (P<0.01).
- The TAP 2A/2B heterozygous genotype was more frequent in cervical cancer patients than in controls (P<0.02).
Conclusions:
- TAP gene mutations are prevalent in cervical carcinomas.
- These findings suggest a potential role for TAP genes in the progression of cervical cancer from precursor lesions.