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Updated: Aug 26, 2026

Assays for Validating Histone Acetyltransferase Inhibitors
Published on: August 6, 2020
Targeted inhibition of V(D)J recombination by a histone methyltransferase
Oleg Osipovich1, Robin Milley, Amber Meade
1Department of Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA.
Abstract:
The tissue- and stage-specific assembly of antigen receptor genes by V(D)J recombination is regulated by changes in the chromatin accessibility of target gene segments. This dynamic remodeling process is coordinated by cis-acting promoters and enhancers, which function as accessibility control elements. The basic epigenetic mechanisms that activate or repress chromatin accessibility to V(D)J recombinase remain unclear. We now demonstrate that a histone methyltransferase overrides accessibility control element function and cripples V(D)J recombination of chromosomal gene segments. The recruited histone methyltransferase induces extensive revisions in the local chromatin environment, including altered histone modifications and de novo methylation of DNA. These findings indicate a key function for histone methyltransferases in the tissue- and stage-specific suppression of antigen receptor gene assembly during lymphocyte development.
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