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5-Lipoxygenase and leukotriene A4 hydrolase expression in primary nephrotic syndrome

A Bakr1, S Hawas, S Slem

  • 1Department of Pediatrics, Mansoura University Children's Hospital, Mansoura, Egypt. ashbakr@mans.edu.eg

Pediatric Nephrology (Berlin, Germany)
|February 27, 2004
PubMed

Insights

Children with active primary nephrotic syndrome (PNS) show gene expression of 5-lipoxygenase (5-LO) and leukotriene A(4) (LTA(4)) hydrolase. These leukotrienes may contribute to PNS but not steroid treatment response in children.

Area of Science:

  • Biochemistry
  • Pediatric Nephrology
  • Molecular Biology

Background:

  • Primary nephrotic syndrome (PNS) is a kidney disorder affecting children.
  • Leukotrienes are implicated in inflammatory processes, but their role in PNS is not fully understood.

Purpose of the Study:

  • To investigate the gene expression of 5-lipoxygenase (5-LO) and leukotriene A(4) (LTA(4)) hydrolase in children with PNS.
  • To determine the association between enzyme expression, disease activity, and steroid responsiveness.

Main Methods:

  • Analysis of 5-LO and LTA(4) hydrolase gene expression in peripheral blood mononuclear cells (PBMN) from children with active PNS, remission, and controls.
  • Utilized reverse transcription and polymerase chain reaction (PCR) for gene amplification.

Main Results:

  • All children with active PNS expressed both 5-LO and LTA(4) hydrolase, unlike controls.
  • A positive correlation was found between proteinuria levels and the expression of both enzymes in active PNS.
  • Enzyme expression did not differ between steroid-sensitive (SSNS) and steroid-resistant (SRNS) PNS patients.

Conclusions:

  • Leukotrienes likely play a role in the pathogenesis of childhood PNS.
  • Leukotriene pathway enzymes do not appear to be involved in the therapeutic response to steroids in pediatric PNS.

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