Related Experiment Video
Updated: Aug 26, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The mitochondrial genome of the olive fly Bactrocera oleae: two haplotypes from distant geographical locations
F Nardi1, A Carapelli, R Dallai
1Department of Evolutionary Biology, University of Siena, Siena, Italy. nardifra@unisi.it
Abstract:
The complete sequence of the olive fly (Bactrocera oleae) mitochondrial genome has been determined. Two independent haplotypes, from flies of distant geographical origin (Italy and Portugal) were completely sequenced. The molecule is 15815 bp long, and shows the gene content and organization typical of insects, namely thirteen protein coding genes (PCGs) encoding proteins involved in oxidative phosphorylation, two rRNAs, twenty-two tRNAs and a long (949 bp) noncoding region. The genomes of the two fly specimens share the same arrangement, differing by a mere thirty-one point mutations. The differences are mostly transitions (26) and synonymous substitutions in PCGs (21). The two new sequences are compared with others already present in the database.
Related Concept Videos
Animal Mitochondrial Genetics
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Export of Mitochondrial and Chloroplast Genes
Evolutionary Relationships through Genome Comparisons
Modern Molecular Taxonomy
Gene Evolution - Fast or Slow?
In contrast, regions which code...

