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Related Experiment Videos

Physiological mechanisms mediating enhanced force generation during development and immune sensitization.

R W Mitchell1, T M Murphy, A R Leff

  • 1Department of Medicine, University of Chicago, IL 60637.

Canadian Journal of Physiology and Pharmacology
|April 1, 1992
PubMed
Summary

Immature swine show greater tracheal smooth muscle (TSM) contraction due to less acetylcholinesterase (AChase) activity. Allergic dogs also exhibit increased TSM contraction, which is normalized by inhibiting AChase.

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Area of Science:

  • Pharmacology
  • Physiology
  • Respiratory Medicine

Background:

  • Acetylcholine (ACh) elicits tracheal smooth muscle (TSM) contraction.
  • Acetylcholinesterase (AChase) regulates ACh activity.
  • Maturation and allergic conditions can alter TSM responsiveness.

Purpose of the Study:

  • To investigate the developmental changes in TSM contraction and AChase activity in swine.
  • To examine the role of AChase in allergic bronchospasm in dogs.
  • To compare TSM responsiveness to ACh in immature versus mature swine and in allergic versus non-allergic dogs.

Main Methods:

  • Isometric tethering of TSM strips at optimal length.
  • Measurement of TSM contraction as a percentage of maximum KCl response (% KCl).

Related Experiment Videos

  • Administration of ACh and the AChase inhibitor physostigmine.

    • Main Results:

    • Maximal ACh-elicited contraction (ATmax) was greater in 2-week-old swine than in 10-week-old swine.
    • Physostigmine augmented ATmax in 10-week-old swine but not in 2-week-old swine, indicating reduced AChase activity in immature animals.
    • TSM from ragweed-sensitized dogs showed augmented efficacy to ACh-elicited contraction compared to controls, an effect diminished by physostigmine.

    Conclusions:

    • Immature swine exhibit increased TSM contraction, partly due to reduced AChase activity.
    • Allergic bronchospasm in dogs involves augmented TSM responsiveness to ACh.
    • AChase plays a significant role in modulating TSM contraction in both developmental and allergic contexts.