Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue microarray (TMA) applications: implications for molecular medicine.

Ronald Simon1, Guido Sauter

  • 1Institute of Pathology, University of Basel, Schoenbeinstrasse 40, CH-4031 Basel, Switzerland. ronald.simon@unibas.ch

Expert Reviews in Molecular Medicine
|February 28, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to: MMR Deficiency is Homogeneous in Pancreatic Carcinoma and Associated with High Density of Cd8-Positive Lymphocytes.

Annals of surgical oncology·2022
Same author

Cytokeratin 7 and cytokeratin 20 expression in cancer: A tissue microarray study on 15,424 cancers.

Experimental and molecular pathology·2022
Same author

Metabolic Syndrome Is Associated with Impaired Survival after Surgery for Pancreatic Neuroendocrine Tumors.

Neuroendocrinology·2022
Same author

VPRBP Functions Downstream of the Androgen Receptor and OGT to Restrict p53 Activation in Prostate Cancer.

Molecular cancer research : MCR·2022
Same author

PITX1 Is a Regulator of TERT Expression in Prostate Cancer with Prognostic Power.

Cancers·2022
Same author

Combination of Biochemical and Cytological Findings for Better Diagnosis in Pleural Effusions.

Advances in experimental medicine and biology·2022
Same journal

Harnessing the Vaginal Microbiome: A New Frontier in the Prevention of Sexually Transmitted Infections.

Expert reviews in molecular medicine·2026
Same journal

Beyond the stomach: Exploring the role of <i>Helicobacter pylori</i> in pushing precancerous lesions to colorectal cancer and the therapeutic potential of probiotics.

Expert reviews in molecular medicine·2026
Same journal

Microchimerism: The Hidden Cellular Dialogue Between Mother and Fetal That Shapes Lifelong Immunity.

Expert reviews in molecular medicine·2026
Same journal

Advances in m6A Modification in Pregnancy-Related Disorders and Female Reproductive Endocrine Diseases.

Expert reviews in molecular medicine·2026
Same journal

Dysregulation of the urea cycle - potential targets for treatment and diagnosis in prostate cancer.

Expert reviews in molecular medicine·2026
Same journal

RAGE-Mediated Signaling in Gynecological Disorders: Review of Molecular Mechanisms and Therapeutic Perspectives.

Expert reviews in molecular medicine·2026
See all related articles

Tissue microarray (TMA) technology enables rapid, cost-effective, and standardized in situ analysis of thousands of clinical tissue samples. This accelerates cancer research and the discovery of genotype-phenotype associations for clinical applications.

Area of Science:

  • Biotechnology
  • Oncology
  • Genomics

Background:

  • High-throughput expression screening platforms like cDNA arrays are crucial for identifying potential drug leads in cancer research.
  • In situ analysis of numerous clinical tissue samples, such as via immunohistochemistry or fluorescence in situ hybridization, is essential for validating candidate genes.
  • Existing methods for analyzing large numbers of tissue samples can be time-consuming and resource-intensive.

Purpose of the Study:

  • To introduce and highlight the advantages of Tissue Microarray (TMA) technology for in situ analysis of clinical tissue samples.
  • To demonstrate how TMA technology can facilitate high-throughput validation of candidate genes identified through expression screening.
  • To emphasize the potential of TMA technology in accelerating the transition of basic research findings to clinical applications.

Related Experiment Videos

Main Methods:

  • Tissue Microarray (TMA) technology involves extracting minute tissue cores (0.6 mm diameter) from up to a thousand conventional paraffin blocks.
  • These tissue cores are re-assembled into a single recipient paraffin block at predefined positions.
  • Sections from the resulting TMA block can then be used for various research analyses, similar to conventional tissue sections.

Main Results:

  • TMA technology allows for the parallel analysis of up to a thousand tissue samples simultaneously.
  • It offers significant cost efficiencies, requiring the same amount of reagents for a thousand samples as for a single large section.
  • The technology ensures standardization, applying identical experimental conditions across all analyzed samples.

Conclusions:

  • TMA technology significantly enhances the speed and efficiency of in situ analysis for large numbers of clinical tissue samples.
  • Its capacity for high statistical power makes it ideal for detecting genotype-phenotype associations.
  • TMA technology is poised to accelerate the translation of basic cancer research into practical clinical applications.