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Updated: Aug 7, 2026
Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Published on: January 6, 2016
Standardization of 32P/33P and 204Tl by liquid scintillation counting
L Rodríguez Barquero1, E García-Toraño, J M Los Arcos
1Laboratorio de Metrología de Radiaciones Ionizantes, CIEMAT, Ed.3, Avda Complutense 22, Madrid 28040, Spain. l.barquero@ciemat.es
Abstract:
Two solutions, one of 32P with a significant contribution of 33P, and another one of 204Tl were standardized by liquid scintillation counting in the frame of two intercomparison exercises organized by the BIPM in 2002. For the 32P solution, the counting efficiencies for 32P and 33P were obtained by the CIEMAT/NIST method. The individual contributions of 32P and 33P were determined by fitting a set of measurements to a function of the half-lives and counting efficiencies of both component nuclides. 204Tl was also standardized by the CIEMAT/NIST method using two counters, three scintillators, HiSafe 3, Insta-Gel Plus and Ultima-Gold, and two different kinds of samples. A total of 480 samples were measured over 45 days with results in full agreement within the uncertainties considered. The efficiency values are also compared to those used in the previous 204Tl intercomparison (1997).
