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Updated: Aug 26, 2026

Real-time Live Imaging of T-cell Signaling Complex Formation
Published on: June 23, 2013
Prion protein is a component of the multimolecular signaling complex involved in T cell activation
Vincenzo Mattei1, Tina Garofalo, Roberta Misasi
1Dipartimento di Medicina Sperimentale e Patologia, Università 'La Sapienza', viale Regina Elena 324, 00161 Rome, Italy.
Abstract:
In this study we analyzed the interaction of prion protein PrP(C) with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrP(C) was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrP(C) is present in microdomains, since it coimmunoprecipitates with GM3 and the raft marker GM1. A strict association between PrP(C) and Fyn was revealed by scanning confocal microscopy and coimmunoprecipitation experiments. The phosphorylation protein ZAP-70 was immunoprecipitated by anti-PrP after T cell activation. These results demonstrate that PrP(C) interacts with ZAP-70, suggesting that PrP(C) is a component of the multimolecular signaling complex within microdomains involved in T cell activation.
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