Related Experiment Video
Updated: Aug 26, 2026
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
Published on: June 28, 2013
Cloning and sequencing of a 16 kDa outer membrane protein gene of Pasteurella multocida P52
P P Goswami1, P Chaudhuri, V Tiwari
1National Biotechnology Centre, Division of Pathology, Indian Veterinary Research Institute, Izatnagar 243 12, U.P., India. ppgnbc@ivri.up.nic.in
Abstract:
The outer membrane proteins (OMPs) of Pasteurella multocida are potential immunogens. The 16 kDa OMP of P. multocida P52, serotype B:2, was identified as one of the major immunodominant antigens. The gene omp16, encoding a 16 kDa outer membrane protein, was amplified, cloned into a pBluescript SK(-) vector and sequenced. Complete sequence homology was observed between the 16 kDa OMP gene of P. multocida P52 (serotypes B:2) and P. multocida T16 (somatic serotype 3,4). This gene was distributed among different serotypes of P. multocida and found to localize in a 6.0 kb HindII fragment of the P. multocida genome.
Insights
Outer membrane proteins (OMPs) of Pasteurella multocida are key antigens. The 16 kDa OMP gene (omp16) from P. multocida P52 shows homology across serotypes, indicating its potential as a vaccine candidate.
Area of Science:
- Veterinary Microbiology
- Bacterial Pathogenesis
- Immunology
Background:
- Outer membrane proteins (OMPs) of Pasteurella multocida are recognized as significant immunogens.
- The 16 kDa OMP from P. multocida P52 (serotype B:2) has been identified as a major immunodominant antigen.
Purpose of the Study:
- To characterize the gene encoding the 16 kDa outer membrane protein (OMP) of Pasteurella multocida.
- To investigate the distribution and genomic localization of the omp16 gene among different P. multocida serotypes.
Main Methods:
- Amplification and cloning of the omp16 gene into a pBluescript SK(-) vector.
- DNA sequencing of the omp16 gene.
- Comparative sequence analysis and genomic mapping.
Main Results:
- The omp16 gene was successfully amplified, cloned, and sequenced.
- Complete sequence homology was found between the 16 kDa OMP gene of P. multocida P52 (serotype B:2) and P. multocida T16 (serotype 3,4).
- The omp16 gene is present in various P. multocida serotypes and is located on a 6.0 kb HindII genomic fragment.
Conclusions:
- The 16 kDa OMP gene (omp16) is conserved across different Pasteurella multocida serotypes.
- The omp16 gene's conservation suggests its potential utility as a target for diagnostic or vaccine development against P. multocida infections.
