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Updated: Jun 24, 2026

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
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A simple method for studying whole sections of rice grain.

Y Ogawa1, W J Orts, G M Glenn

  • 1USDA, ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|March 3, 2004
PubMed
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This summary is machine-generated.

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Researchers developed a simple adhesive tape method for collecting flat, whole brown rice kernel sections. This technique, combined with autofluorescence, clearly visualizes cell walls for histological analysis.

Area of Science:

  • Plant Science
  • Materials Science
  • Microscopy

Background:

  • Histological investigation of whole rice kernels is crucial for understanding grain quality and development.
  • Traditional sectioning methods can lead to compressed or damaged samples, hindering detailed analysis.

Purpose of the Study:

  • To develop a novel, simple, and effective method for collecting intact histological sections of whole brown rice kernels.
  • To enable clear visualization of cellular structures, particularly cell walls, within the entire rice kernel.

Main Methods:

  • A dehydration series using ethanol and xylene for tissue preparation.
  • Paraffin embedding of the prepared rice kernels.
  • Utilizing a rotary microtome with a specialized adhesive tape to collect intact, flat sections.

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  • Leveraging autofluorescence properties for cell wall visualization.

    • Main Results:

    • Successfully obtained flat, whole longitudinal and transverse sections of brown rice kernels.
    • The adhesive tape method prevented section compression and damage.
    • Autofluorescence clearly delineated cell walls throughout the entire kernel sections.
    • Scanning electron microscopy confirmed the high quality and integrity of the prepared sections.

    Conclusions:

    • The developed adhesive tape technique offers a simple and efficient approach for whole rice kernel histology.
    • This method significantly improves the ability to study rice kernel cellular structures and properties.
    • The technique is valuable for research in plant biology, grain science, and quality assessment.