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[Studies on DNA damage in workers with long-term exposure to lower concentration of carbon disulfide]
Xiao-qing Chen1, Xiao-dong Tan
1Department of Hygiene, School of Public Health, Wuhan University, Hubei 430071, China.
Objective:
DNA damage in human buccal cells of workers occupationally long-term exposed to carbon disulfide (CS(2)) was monitored with comet assay.
Methods:
Ninety workers exposed to CS(2) were randomly selected as exposure group from a large-scale chemical fiber manufacturer in Hubei and 81 workers not exposed to CS(2) as control group. DNA damage in their buccal cells was detected with comet assay.
Results:
Rate of DNA tail was 0.51% in exposure group, significantly higher than that in control group (0.23%), with very statistical significance. Rate of DNA tail was 0.50% in male exposure group and 0.56% in exposure group with short length of employment, significantly higher than that in control group (0.08% and 0.25%, respectively). Multivariate unconditional logistic regression analysis showed that possibility of DNA damage was significantly higher in exposure group than that in control group (P < 0.05).
Conclusion:
Long-term exposure to lower concentration of carbon disulfide could cause certain damage to human buccal cell DNA.
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