Related Experiment Video
Updated: Aug 16, 2026
Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains
Published on: June 3, 2020
[Clinical diagnosis of 26 cases of brain death]
Tian-xi Zhang1, Yi-jue Shi, Hui-qiu Sheng
1Department of Neurosurgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200050, China.
Objective:
To explore clinical diagnostic criterion of brain death.
Methods:
Evaluation was made on 26 cases of brain death through routine check-up such as brain stem reflex, GCS, pupil dilatation and limbs reflex. Among whom apnea test was made in 10 cases and EEG-Holter monitoring in 5 cases. Different dose of 1 mg, 2 mg, 5 mg Atropine test in 21 cases of brain death were administered to compare with the result in another 15 cases of deep coma patients.
Results:
The positive of apnea test has some relation with the mode of respirator executed. The negative of Atropine test in brain death group has significant difference with that of deep coma group (P < 0.05). EEG-Holter is valuable in determining the exact time of brain death. Pupil changes including mydriasis 14 cases, miosis 2 cases and inequality of pupil 5 cases. Limbs reflex was preserved in 5 cases.
Conclusions:
A protocol for executing apnea is recommended. Atropine 1 mg should be the first dosage when executing Atropine test. Dynamic EEG has forensic value while mydriasis and loss of limbs reflex should not be regarded as characteristic sign of brain death.
