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Published on: October 23, 2018
Determination of volatile anesthetics isoflurane and enflurane in mouse brain tissues using gas chromatography-mass
Xin-Sheng Deng1, Victoria J Simpson
1Department of Pharmacology, University of Colorado Health Sciences Center, Mail Stop C-236, 4200 E. 9th Avenue, Denver, CO 80262, USA. xin-sheng.Deng@uchsc.edu
Introduction:
A method for determination of the volatile anesthetics, isoflurane, and enflurane in mouse brain tissues using headspace gas chromatography-mass spectrometry (GC-MS) is described.
Methods:
Halothane was used as internal standard (I.S.). Brain samples were completely homogenized in ice-cold water and isoflurane, enflurane, and I.S. were extracted with headspace. One milliliter of headspace gas was injected onto the GC-MS and separation was achieved by using porous layer open tubular (PLOT) capillary column with a solid stationary phase (GSC). As a result, isoflurane, enflurane, and halothane were cleanly separated.
Results:
The method demonstrated satisfactory recovery (72% and 76% for isoflurane and enflurane, respectively) and linear calibration ranges of 0.015-2.20 and 0.0152-3.94 microg/sample for isoflurane and enflurane, respectively. Reproducibility calculated as CV% was 3.3-3.9% for all intraday and interday determinations. The procedure was applied for quantitation of isoflurane and enflurane in about 300 mouse brain samples for genetic behavioral study.
Discussion:
The method was achieved and shown to be effective.
