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Experimental calcification in rat submandibular gland.

Y Sakuma1, M Mori

  • 1Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Gifu, Japan.

Cellular and Molecular Biology
|July 1, 1992
PubMed
Summary

This study details calciphylaxis, a rapid calcification in rat salivary glands induced by dihydrotachysterol (DHT) and 5-hydroxytryptamine (5-HT). Findings reveal distinct calcification types and compositions, suggesting dystrophic calcification.

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Area of Science:

  • Pathology
  • Mineral Metabolism
  • Biomaterials Science

Background:

  • Calciphylaxis is a severe condition characterized by rapid tissue calcification.
  • Understanding the mechanisms and composition of calcified lesions is crucial for potential therapeutic strategies.

Purpose of the Study:

  • To investigate the histopathological and biophysical characteristics of calciphylaxis in rat submandibular glands.
  • To analyze the composition and structure of calcified deposits using advanced imaging and diffraction techniques.

Main Methods:

  • Induction of calciphylaxis using dihydrotachysterol (DHT) and 5-hydroxytryptamine (5-HT) in rats.
  • Light and electron microscopy, histochemistry, X-ray microanalysis (XMA), and X-ray powder diffraction.
  • Lectin staining (Con A, UEA-1) to identify matrix components.

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Main Results:

  • Three distinct types of calcified lesions were identified histologically.
  • Calcification matrices contained mannose, fucose, and glucose, with lectin staining indicating these components.
  • XMA revealed magnesium whitelockite patterns and high Ca, P, Mg ions in type 3 lesions. X-ray diffraction confirmed hydroxyapatite formation.

Conclusions:

  • The calcified lesions in rat salivary glands exhibit characteristics of dystrophic calcification.
  • Necrotic alterations induced by hypercalcemia are implicated in the pathogenesis of these calcifications.
  • The study provides detailed insights into the composition and structure of calciphylaxis-induced mineral deposits.