Related Experiment Video
Updated: Aug 26, 2026

Identification of Plasmodesmal Localization Sequences in Proteins In Planta
Published on: August 15, 2017
Interaction between a geminivirus replication protein and the plant sumoylation system
A G Castillo1, L J Kong, L Hanley-Bowdoin
1Department of Cellular Biology, Genetics and Animal Physiology, Málaga University, Málaga 29071, Spain.
Abstract:
Geminiviruses are small DNA viruses that replicate in nuclei of infected plant cells after accumulation of host replication machinery. Tomato golden mosaic virus (TGMV) and Tomato yellow leaf curl Sardinia virus (TYLCSV) encode a protein, RepAC1 (or Rep), that is essential for viral replication. Rep/RepAC1 is an oligomeric protein that binds to double-stranded DNA, catalyzes cleavage and ligation of single-stranded DNA, and is sufficient for host induction. It also interacts with several host proteins, including the cell cycle regulator, retinoblastoma, and essential components of the cell DNA replication machinery, like proliferating nuclear cell antigen (PCNA) and RFC-1. To identify other cellular proteins that interact with Rep/RepAC1 protein, a Nicotiana benthamiana cDNA library was screened with a yeast two-hybrid assay. The host cell sumoylation enzyme, NbSCE1 (N. benthamiana SUMO-conjugating enzyme, homolog to Saccharomyces cerevisiae UBC9), was found to interact specifically with RepAC1. Mapping studies localized the interaction to the N-terminal half of RepAC1. Effects on geminivirus replication were observed in transgenic plants with altered levels of SUMO, the substrate for UBC9.
Related Concept Videos
Subviral Agents
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Inhibitors of Viral Protein Synthesis
Microbe-Plant Interactions
Protein Complex Assembly
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Leaky Scanning

