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Interaction between a geminivirus replication protein and the plant sumoylation system
A G Castillo1, L J Kong, L Hanley-Bowdoin
1Department of Cellular Biology, Genetics and Animal Physiology, Málaga University, Málaga 29071, Spain.
Journal of Virology
|March 3, 2004
Summary
Geminivirus replication involves the RepAC1 protein interacting with host factors. This study identifies a new interaction with the SUMO-conjugating enzyme NbSCE1, impacting viral replication.
Area of Science:
- Plant virology
- Molecular biology
- Biochemistry
Background:
- Geminiviruses are plant pathogens requiring host replication machinery.
- The RepAC1 protein is crucial for geminivirus DNA replication and interacts with host proteins like PCNA and RFC-1.
Purpose of the Study:
- To identify novel host cellular proteins interacting with the geminivirus RepAC1 protein.
- To investigate the role of sumoylation in geminivirus replication.
Main Methods:
- Yeast two-hybrid screening of a Nicotiana benthamiana cDNA library.
- Interaction mapping of RepAC1.
- Analysis of geminivirus replication in transgenic plants with altered SUMO levels.
Main Results:
- The geminivirus RepAC1 protein specifically interacts with NbSCE1, a SUMO-conjugating enzyme.
- The interaction site was mapped to the N-terminal half of RepAC1.
- Altered SUMO levels in transgenic plants affected geminivirus replication.
Conclusions:
- NbSCE1 is a novel host factor that interacts with geminivirus RepAC1.
- Sumoylation may play a role in regulating geminivirus replication.