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Dialysis-Chelex method for determination of exchangeable copper in human plasma
Tony I Venelinov1, Ian M Davies, John H Beattie
1Department of Analytical Chemistry, Faculty of Chemistry, Sofia University, 1 J. Bourchier Blvd, 1164 Sofia, Bulgaria.
Abstract:
A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.