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Garlic metabolites fail to inhibit the activation of the transcription factor NF-kappaB and subsequent expression of

Verena M Dirsch¹, Hans-Peter Keiss, Angelika M Vollmar

¹Dept. of Pharmacy, Center of Drug Research, Butenandtstr. 5-13, 81377, Munich, Germany. verena.dirsch@cup.uni-muenchen.de

European Journal of Nutrition

|March 3, 2004

Summary

This summary is machine-generated.

Garlic metabolites diallyldisulfide (DADS) and allylmercaptane (AM) do not inhibit NF-kappaB activation or E-selectin expression in endothelial cells. This suggests NF-kappaB is not a primary target for these garlic compounds in preventing atherosclerosis.

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Area of Science:

Cardiovascular Research
Molecular Biology
Nutraceutical Science

Background:

Atherogenesis involves NF-kappaB-regulated proinflammatory gene products like adhesion molecules.
Garlic consumption is linked to antiatherogenic effects, but its molecular mechanisms are unclear.

Purpose of the Study:

To investigate if garlic metabolites diallyldisulfide (DADS) and allylmercaptane (AM) affect TNF-alpha-induced NF-kappaB activation.
To determine if DADS and AM influence the expression of NF-kappaB-regulated E-selectin in human umbilical endothelial cells (HUVECs).

Main Methods:

NF-kappaB DNA binding activity was assessed using electrophoretic mobility shift assay (EMSA).
NF-kappaB transactivation activity was measured via a luciferase reporter gene assay.
E-selectin expression was quantified by flow cytometry.

Main Results:

DADS and AM did not inhibit TNF-alpha-induced NF-kappaB DNA binding or transactivation.
The expression of E-selectin, an NF-kappaB-regulated gene, was not reduced by DADS or AM treatment.

Conclusions:

NF-kappaB does not appear to be a major molecular target for the antiatherogenic effects of DADS and AM.
The antiatherogenic properties of garlic may involve pathways independent of NF-kappaB modulation by these specific metabolites.