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S1 glycoprotein gene analysis of infectious bronchitis viruses isolated in Korea

S K Lee1, H W Sung, H M Kwon

  • 1Department of Veterinary Medicine, Kangwon National University, Chunchon, Republic of Korea.

Archives of Virology
|March 3, 2004
PubMed

Insights

Infectious bronchitis virus (IBV) in Korea shows significant genetic diversity. Analysis of S1 glycoprotein genes revealed unique clusters, indicating ongoing evolution and co-circulation of IBV strains in the region.

Area of Science:

  • Veterinary Virology
  • Molecular Epidemiology
  • Avian Infectious Diseases

Background:

  • Infectious bronchitis virus (IBV) is a significant pathogen affecting poultry worldwide.
  • Understanding the genetic diversity of IBV is crucial for developing effective control strategies.

Purpose of the Study:

  • To investigate the genetic characteristics of IBV isolates from suspected cases in Korea.
  • To analyze the genetic diversity of the S1 glycoprotein gene among Korean IBV isolates.

Main Methods:

  • Isolation and identification of IBV from clinical samples (kidney, trachea, cecal tonsil).
  • Amplification of the S1 glycoprotein gene using reverse transcriptase - polymerase chain reaction (RT-PCR).
  • Restriction fragment length polymorphism (RFLP) analysis, gene sequencing, and phylogenetic tree construction.

Main Results:

  • Fifteen Korean IBV isolates were classified into 4 groups by RFLP patterns.
  • Ten isolates shared the KM91 RFLP pattern, while others matched Arkansas, Connecticut, and Massachusetts strains.
  • Phylogenetic analysis revealed 5 clusters, with 3 distinct from non-Korean strains and a separate cluster for specific Korean isolates (K069-01, K507-01, K774-01, K142-02).
  • Amino acid sequence similarity ranged from 42.9% to 96.5% compared to non-Korean strains.

Conclusions:

  • Korean IBV isolates exhibit considerable genetic diversity.
  • The findings suggest ongoing evolution and co-circulation of distinct IBV strains within Korea.
  • These genetic variations may impact vaccine efficacy and disease management.

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