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Updated: Aug 26, 2026
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Published on: December 1, 2020
[Structure-based design, synthesis and evaluation of bioactivity of anti-P-gp peptide mimetic]
Jing Qi1, Hui Peng, Ying-dai Gao
1State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300020, China.
Aim:
To design and evaluate the small peptide mimetic of anti-P-glycoprotein (P-gp) antibody (PHMA02).
Methods:
From the three dementional structure analysis of computer modeling of PHMA02 CDR loops, a small peptide mimetic was designed and determined by flow cytometry.
Results:
Anti-P-gp peptide mimetic functionally similar to PHMA02 was developed. The peptide mimetic competitively inhibits PHMA02 binding to P-gp and partially block the P-gp function as a drug efflux pump in K562/A02 cells.
Conclusion:
Some special conformational properties of CDR loops of antibody might serve as lead structures for develop new biological peptide mimetics. Antibody-structure-based design would develop new drug in the future.
