Related Experiment Videos
[Secondary structure of insulin encapsulated within liposomes]
Xuan Zhang1, Li-xin Huang, Song-qing Nie
1Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Aim:
To determine the secondary structure of insulin encapsulated within liposomes.
Methods:
The secondary structure of insulin, mixture of insulin with liposomes (I) and insulin liposomes (II) were determined by Fourier transform infrared (FTIR) spectroscopy.
Results:
The figures of secondary structure of insulin, sample I and II were similar. The results showed that the amount of alpha-helix and beta-sheet of insulin, sample I and II had little difference, from 36.09% (insulin) to 31.68% (sample I) and 31.45% (sample II), and from 47.83% (insulin) to 53.29% (I) and 51.36% (II), respectively. The results showed that the insulin of sample I and II did not insert in liposomes membrane, only adsorbed or extendedon the surface of the liposomes.
Conclusion:
The secondary structure of insulin encapsulated within liposomes has not been destroyed and still remain the original state.