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Published on: December 9, 2016
A database designed to computationally aid an experimental approach to alternative splicing.
C L Zheng1, T M Nair, M Gribskov
1University of California, San Diego, San Diego Supercomputer Center, 9500 Gilman Dr., La Jolla, CA 92093, USA. czheng@sdsc.edu
Summary
Researchers developed the Manually Annotated Alternatively Spliced Events (MAASE) database to aid in profiling alternative splicing. This resource supports experimentalists by providing access to annotated alternative splicing events (ASEs) for microarray design.
Area of Science:
- Molecular Biology
- Bioinformatics
Background:
- Alternative splicing is a key mechanism regulating gene expression.
- Profiling alternative splicing events (ASEs) is crucial for understanding cellular processes.
- Existing resources for ASEs may lack experimentalist-focused features.
Purpose of the Study:
- To develop a comprehensive and user-friendly resource for alternative splicing information.
- To support the design and execution of microarray experiments for alternative splicing analysis.
- To create a scalable platform for the acquisition and contribution of annotated ASEs.
Main Methods:
- Development of the Manually Annotated Alternatively Spliced Events (MAASE) database system.
- Integration of a web-based annotation system combining manual and computational approaches.
- Creation of a curated database for querying annotated ASEs and associated sequences.
Main Results:
- The MAASE database provides convenient online access to annotated ASEs.
- The annotation system facilitates both identification and contribution of ASEs.
- The database offers access to junctional and surrounding sequences for experimental design.
Conclusions:
- MAASE is a valuable resource for experimentalists studying alternative splicing.
- The combined manual and computational annotation approach ensures a comprehensive collection of ASEs.
- MAASE supports the advancement of microarray-based alternative splicing profiling.
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