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Related Experiment Videos

Cyclin F disruption compromises placental development and affects normal cell cycle execution.

Michael T Tetzlaff1, Chang Bai, Milton Finegold

  • 1Department of Human and Molecular Genetics, Texas Children's Hospital, Baylor College of Medicine, Houston, Texas 77030, USA.

Molecular and Cellular Biology
|March 3, 2004
PubMed
Summary

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Mice lacking cyclin F (CycF) die early due to developmental defects, primarily in placental formation. However, mouse embryonic fibroblasts deficient in cyclin F show cell cycle regulation issues, indicating its role in cell cycle control.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • Human cyclin F, identified by its ability to suppress a yeast cell cycle mutant, is highly conserved and contains an F-box domain.
  • Its structure suggests a role in proteolysis via SCF complex formation, coordinating critical cell cycle events.

Purpose of the Study:

  • To investigate the in vivo function of cyclin F during mammalian development and cell cycle regulation.
  • To determine the consequences of cyclin F deficiency on embryonic development and cellular processes.

Main Methods:

  • Generation of cyclin F knockout mice and conditionally deficient mouse embryonic fibroblasts (MEFs).
  • Analysis of developmental phenotypes, including placental formation and tissue-specific deletion.
  • Assessment of cell cycle progression defects in cyclin F-deficient MEFs.

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Main Results:

  • Cyclin F deficient (CycF-/-) mice exhibit severe developmental anomalies and embryonic lethality around day 10.5, linked to placental failures.
  • Tissue-specific deletion showed cyclin F is dispensable for many embryonic and adult tissues.
  • MEFs lacking cyclin F display viable but impaired cell cycle progression, including slower doubling times and delayed reentry from quiescence.

Conclusions:

  • Cyclin F is essential for embryonic development, particularly placental formation, and its absence leads to lethality.
  • While not critical for all tissue development, cyclin F plays a significant role in regulating cell cycle progression and quiescence reentry in fibroblasts.