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Updated: Aug 12, 2026

Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter Assay Platforms
Published on: January 25, 2018
Gamma-secretase: a catalyst of Alzheimer disease and signal transduction
1Department of Biological Chemistry, Merck Research Laboratories, West Point, PA 19486, USA. yueming_li@merck.com
Abstract:
Gamma-secretase is a membrane-bound protease that cleaves within the transmembrane region of the amyloid precursor protein to generate the C termini of both major peptides that constitute the amyloid plaques found in Alzheimer disease (AD). Recent biochemical studies provide compelling evidence that presenilins, membrane-spanning proteins associated with early-onset familial AD, are novel aspartyl proteased that function within a macromolecular complex to mediate gamma-secretase activity. These new insights underscore the role of the presenilins in regulated intramembrane proteolysis and could possibly lead to the inhibition of gamma-secretase activity as a therapy for AD.
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