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Updated: Jul 2, 2026
Isolation, Processing and Analysis of Murine Gingival Cells
Published on: July 2, 2013
[Effects of bFGF on biological characteristics of cells derived from periodontium]
1Hospital of Stomatology attached to Shangdong Medical University, Shandong 250012, China.
Objective:
To observe effects of basic fibroblast growth factor on the biological characteristics of cultured human gingival,periodontal ligament fibroblasts and human alveolar bone cells, such as proliferation, alkaline phosphatase activity, protein synthesis and the formation of mineralized nodules.
Methods:
Using cell culture technique, MTT colorimetric assay, ALP activity assay, Commasie brilliant blue staining and Dahl McGec-Russell's alizarin red stain for calcium.
Results:
bFGF enhanced the proliferative responses of the three types of cells. In contrast, bFGF inhabited the induction of alkaline phosphatase activity, protein synthesis and the mineralized nodule formation by PDLF and ABC.
Conclusion:
bFGF can enhance cell proliferation while inhibit cytodifferentiation, thus accelerating periodontal regeneration.
