Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Effect of cholecystectomy on gallbladder bile composition.

S A Ahrendt1, T H Magnuson, H A Pitt

  • 1Department of Surgery, The Johns Hopkins Medical Institutions, Baltimore, Maryland.

Digestive Diseases and Sciences
|August 1, 1992
PubMed
Summary

Bile composition changes after gallbladder manipulation during surgery. Consistent sampling techniques are crucial for accurate biliary research findings.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of Klebsiella pneumoniae and quinolone treatment on prognosis in patients with pancreatic cancer.

The British journal of surgery·2021
Same author

Risk of malignancy in small pancreatic cysts decreases over time.

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]·2020
Same author

An elevated CA 19-9 is associated with invasive cancer and worse survival in IPMN.

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]·2020
Same author

Intraoperative Fluid Administration and Surgical Outcomes Following Pancreaticoduodenectomy: External Validation at a Tertiary Referral Center.

World journal of surgery·2018
Same author

Major Complications Independently Increase Long-Term Mortality After Pancreatoduodenectomy for Cancer.

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract·2018
Same author

Cytoreductive surgery and HIPEC improve survival compared to palliative chemotherapy for biliary carcinoma with peritoneal metastasis: A multi-institutional cohort from PSOGI and BIG RENAPE groups.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2018

Area of Science:

  • Gastroenterology and Hepatology
  • Surgical Research

Background:

  • Human bile samples are vital for biliary research.
  • Optimal methods for collecting bile samples remain unclear.

Purpose of the Study:

  • To investigate if bile composition differs based on sampling time relative to gallbladder manipulation during cholecystectomy.
  • To establish best practices for bile sample collection in surgical research.

Main Methods:

  • Collected paired gallbladder bile samples from 26 patients undergoing cholecystectomy.
  • Obtained initial samples before gallbladder manipulation and second samples post-excision.
  • Analyzed differences in pH, total protein, phospholipid, cholesterol, bile acids, bilirubin, calcium, cholesterol saturation index, and total lipids.

Related Experiment Videos

Main Results:

  • Post-cholecystectomy samples showed significantly higher pH and total protein.
  • Bile obtained after surgery had significantly lower phospholipid content.
  • Cholesterol, bile acids, bilirubin, calcium, cholesterol saturation index, and lipids remained consistent between sampling methods.

Conclusions:

  • Gallbladder manipulation during cholecystectomy alters bile composition.
  • Standardized bile sampling techniques are essential for reliable biliary research.