Related Experiment Video
Updated: Aug 26, 2026

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
Published on: March 9, 2010
Human reticulon 1-A and 1-B interact with a medium chain of the AP-2 adaptor complex
1Department of Virology, Kurume University School of Medicine, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan. iwahashi@med.kurume-u.ac.jp
Abstract:
Human reticulon family gene 1 (RTN1) is expressed predominantly in neuroendocrine tissues, and produces three proteins termed RTN1-A, RTN1-B, and RTN1-C. Yeast two-hybrid screening indicated that RTN1-A and RTN1-B interacted with AP50, a component of the AP-2 adaptor complex involved in endocytosis. In contrast, RTN1-C did not interact with AP50. Three RTN1 proteins contain the same C-terminal domain. In addition, RTN1-A and RTN1-B share N-terminal 168 amino acid region, suggesting that the 168 amino acid region might play a role in regulating the endocytic process. Although no apparent morphological change of the endocytic organelles was observed, the association of AP50 with the internalized clathrin-coated vesicles was moderately affected when CV-1 cells were directed to express stably RTN1-A or RTN1-B.
Related Concept Videos
Rab Proteins
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
The ADP/ATP Carrier Protein
Rab Cascades
Protein Translocation Machinery on the ER Membrane
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Cotranslational Protein Translocation
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Directing Proteins to the Rough Endoplasmic Reticulum

