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Functional characteristics of photochemically treated platelets.
Gerard A J Jansen1, Huub H D M van Vliet, Hans Vermeij
1Sanquin Blood Bank South West Region, Wytemaweg 10, 3015 CN Rotterdam, the Netherlands.
Transfusion
|March 5, 2004
Summary
Photochemical treatment (PCT) using the T-bag S59 Reduction Device significantly reduced platelet function. The Wafer S59 Reduction Device showed minimal impact on platelet quality and function in vitro.
Area of Science:
- Blood product safety
- Transfusion medicine
- Photochemistry
Background:
- Photochemical treatment (PCT) with amotosalen HCl (S59) and UVA light inactivates pathogens and WBCs.
- PCT aims to enhance blood product safety.
- This study investigates PCT's in vitro effects on platelet function.
Purpose of the Study:
- To evaluate the impact of PCT on platelet functional characteristics.
- To compare the effects of two different PCT systems (T-bag SRD and Wafer SRD) on platelets.
- To assess changes in platelet quality, function, apoptosis, and lysis post-PCT.
Main Methods:
- Platelet concentrates were treated with T-bag SRD, Wafer SRD, or served as controls.
- Evaluated product quality (HSR, pH), platelet function (aggregation, activation), apoptosis (annexin V, caspase 3), and lysis.
- Utilized in vitro assays to assess functional parameters.
Main Results:
- No significant differences in product quality, P-selectin expression, or apoptosis markers were observed between groups.
- PCT with the T-bag SRD significantly decreased platelet aggregation capacity and increased plasma LDH.
- The Wafer SRD showed no significant impact on platelet aggregation or LDH levels.
Conclusions:
- PCT using the experimental T-bag SRD significantly impairs platelet function.
- The commercially available Wafer SRD demonstrates minimal in vitro effects on platelet quality and function.
- Wafer SRD appears to be a safer option for photochemical pathogen inactivation of platelets.