Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nerve growth factor potentiates actomyosin adenosinetriphosphatase.

P Calissano, G Monaco, L Castellani

    Proceedings of the National Academy of Sciences of the United States of America
    |May 1, 1978
    PubMed
    Summary

    Nerve growth factor (NGF) promotes actin polymerization and myosin ATPase activation, forming paracrystalline structures. Heavy meromyosin displaces NGF, suggesting a self-regulatory mechanism for NGF action in cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Corrigendum to: "Extreme ultraviolet transient gratings: A tool for nanoscale photoacoustics" [Photoacoustics 29 (2023) 100453].

    Photoacoustics·2025
    Same author

    Drawing improves memory in patients with hippocampal damage.

    Memory & cognition·2024
    Same author

    Origin-Independent Dynamic Polarizability Density from Coupled Cluster Response Theory.

    Journal of chemical theory and computation·2023
    Same author

    Extreme ultraviolet transient gratings: A tool for nanoscale photoacoustics.

    Photoacoustics·2023
    Same author

    Percutaneous ethanol sclerotherapy is a promising treatment for recalcitrant angiolymphoid hyperplasia with eosinophilia.

    Clinical and experimental dermatology·2021
    Same author

    Transdermal nicotine in non-smokers: A systematic review to design COVID-19 clinical trials.

    Respiratory medicine and research·2021

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Neuroscience

    Background:

    • Nerve growth factor (NGF) is crucial for neuronal development and survival.
    • Actin cytoskeleton dynamics are fundamental to cellular processes.
    • Myosin ATPase activity drives muscle contraction and other cellular movements.

    Purpose of the Study:

    • To investigate the interaction between NGF and actin.
    • To elucidate the role of NGF in actin polymerization and organization.
    • To understand how NGF influences myosin ATPase activity.

    Main Methods:

    • Binding studies to determine stoichiometry of NGF-actin complexes.
    • Polymerization assays to observe actin organization.
    • Enzyme kinetics to measure myosin ATPase activation.

    Related Experiment Videos

  • Microscopy to visualize paracrystalline structures and heavy meromyosin decoration.

    • Main Results:

    • NGF promotes actin polymerization and formation of paracrystalline structures.
    • NGF enhances myosin ATPase activation beyond that of actin alone.
    • Heavy meromyosin displaces NGF from actin filaments, particularly in the absence of ATP.
    • Paracrystalline structures are disrupted by heavy meromyosin binding.

    Conclusions:

    • NGF modulates actin organization and myosin activity through a novel mechanism.
    • A self-regulatory, self-propagating cycle of NGF-actin-myosin interaction is proposed.
    • This interaction may be key to NGF's signaling in target cells.