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Doxepin protects cultured neurons against oxidative stress-induced injury
Bian-sheng Ji1, He Ji, Guo-qing Liu
1Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China.
Aim:
To investigate protective effects of doxepin (Dox) on cultured neuronal injury induced by oxidative stress.
Methods:
Cytotoxicities of glutamate (Glu 0.5 mmol/L, 15 min), sodium dithionite (0.5 mmol/L, 24 h) or hemoglobin (Hb100 mg/L,24 h)and the protective effects of Dox were observed.
Results:
Exposure of cultured neurons to Glu, sodium dithionite and Hb developed a neurotoxicity expressed in the thiazol blue tetrazolium bromide (MTT) assay, the increase of lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) content and intracellular [Ca2+]i accumulation, as well as the decrease of superoxide dismutase (SOD) activity. DOX 1-100 nmol/L significantly inhibited all above changes.
Conclusion:
Dox protects cultured neurons against oxidative stress-induced injury by suppressing intracellular [Ca2+]i accumulation, decreasing lipid peroxide generation and stimulating antioxidant enzyme.
