Related Experiment Video
Updated: Aug 26, 2026
Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death
Published on: October 21, 2012
Identification of genes responsive to apoptosis in HL-60 cells
Wei Jin1, Le-feng Qu, Ping Min
1Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Aim:
To identify genes responsive to apoptosis in HL-60 cells treated by homoharringtonine.
Methods:
cDNA microarray technology was used to detect gene expression and the result of microarrays for genes (TIEG and VDUP1) was confirmed by Northern analysis.
Results:
Seventy-five individual mRNAs whose mass changed significantly were identified. Among these genes (25 were up-regulated and 50 were down-regulated), most are known related to oncogenes and tumor suppressor. Some genes were involved in apoptosis signaling pathways.
Conclusion:
TGF beta and TNF apoptosis signaling pathways were initiated during apoptosis in HL-60 cells. TIEG and VDUP1 play important roles in mediating apoptosis.
Related Concept Videos
01:31The Intrinsic Apoptotic Pathway
01:30Apoptosis