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Updated: Aug 11, 2026

Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis
Published on: March 25, 2014
Nuclear polarity and nuclear trafficking in Drosophila
1Edinburgh University, Institute of Cell and Molecular Biology, Swann Building, King's Buildings, Mayfield Road, Edinburgh, EH9 3JR, Scotland, UK.
Abstract:
There has been considerable progress in understanding the mechanism of movement of proteins and RNA between the nucleus and cytoplasm in single cells and in vitro. Recently, some components of the nuclear trafficking machinery have also been identified in Drosophila melanogaster allowing the study of phenotypes of mutant trafficking genes during multicellular development. Furthermore,Drosophila has highly polarized cells at a number of different developmental stages and many asymmetrically localized transcripts and proteins that allow the study of nuclear polarity and orientation and its role in mRNA export and cytoplasmic localization.
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