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Studies on structural and immunogenic polypeptides of hydropericardium syndrome virus by SDS-PAGE and western

Rajesh Kumar1, Rajesh Chandra

  • 1Department of Veterinary Microbiology, College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar 263 145, U.S. Nagar, Uttaranchal, India. rajeshvet@rediffmail.com

Comparative Immunology, Microbiology and Infectious Diseases
|March 6, 2004
PubMed

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Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.