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Related Experiment Videos

Biological imaging with a near-field optical setup.

Morgan Denyer1, Ruggero Micheletto, Ken Nakajima

  • 1School of Pharmacy, University of Bradford, Bradford, United Kingdom.

Journal of Nanoscience and Nanotechnology
|March 9, 2004
PubMed
Summary

Custom-built scanning near-field optical microscope (SNOM) systems image biological samples. This study demonstrates SNOM

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Area of Science:

  • Biophysics
  • Optical Microscopy
  • Cell Biology

Background:

  • Scanning near-field optical microscopy (SNOM) offers high resolution for sample imaging.
  • SNOM systems are increasingly applied to biological sample analysis.
  • Existing SNOM systems have limitations in imaging dynamic biological processes.

Purpose of the Study:

  • To develop and demonstrate a custom-built SNOM system for biological applications.
  • To evaluate the potential of near-field optical examination for biological materials.
  • To image both fixed and live whole-cell samples in various environments.

Main Methods:

  • Construction of a noncontact scanning near-field optical microscope (SNOM).
  • Imaging of fixed whole-cell samples in air and liquid.
  • Imaging of live whole-cell samples in liquid media.

Main Results:

  • Successfully imaged fixed and live whole-cell samples using the custom SNOM.
  • Achieved subnanometric resolution in the z-axis for monitoring live cell dynamics.
  • Demonstrated fluorescent imaging capabilities for whole cells in liquid.

Conclusions:

  • Custom SNOM systems are viable for near-field optical examination of biological samples.
  • SNOM can monitor live cell dynamics and perform fluorescent imaging in liquid.
  • Further development can enhance SNOM resolution for biological applications.

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