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Updated: Aug 26, 2026

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification
Published on: February 27, 2026
Comparison of preparative HPLC/MS and preparative SFC techniques for the high-throughput purification of compound
Philip A Searle1, Keithney A Glass, Jill E Hochlowski
1Medicinal Chemistry Technologies, Global Pharmaceutical Research & Development, Abbott Laboratories, Department R4CP, Building AP10, 100 Abbott Park Road, Abbott Park, IL 60064-6101, USA. philip.searle@abbott.com
Abstract:
A diverse set of 16 high-throughput organic synthesis libraries, consisting of 48 samples per library, has been purified by both preparative supercritical fluid chromatography (SFC) and preparative high-performance liquid chromatography (HPLC). This paper details the relative effectiveness of these two purification techniques in terms of success, yield, and purity of final product.
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