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Updated: Aug 26, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
Efficient production of recombinant human transcription factor IIE
Woo J Moon1, Jessica A Apostol, Aaron J McBride
1Department of Biochemistry and Molecular Biology, Michigan State University, E. Lansing, MI 48824-1319, USA.
Abstract:
Transcription factor IIE (TFIIE) is a general initiation and promoter escape factor for RNA polymerase II composed of p56 (TFIIE-alpha) and p34 (TFIIE-beta) subunits. Our laboratories experienced difficulty producing adequate quantities of recombinant human TFIIE-alpha for in vitro studies using available clones. We therefore re-engineered the TFIIE subunit production vectors and tested various Escherichia coli host strains to optimize expression. We report a much-improved system for production of pure, soluble, and active TFIIE complex for in vitro studies.
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