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Updated: Aug 26, 2026
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
Published on: July 29, 2018
Initial human myeloid/dendritic cell progenitors identified by absence of myeloperoxidase protein expression
Walter Scholz1, Barbara Platzer, Angela Schumich
1Institute of Immunology, University of Vienna, Vienna, Austria.
Abstract:
Myeloperoxidase (MPO) represents an early-appearing and highly reliable intracellular myeloid lineage marker molecule. MPO protein can be detected in a subset of human hematopoietic bone marrow progenitor cells and in granulomonopoietic (GM) cells. However, other myeloid-related cell types such as epidermal Langerhans-type dendritic cells (LC) lack MPO. Therefore, human myeloid progenitors might be subdivided based on MPO protein expression into functional subsets. Here we identified two consecutive myelopoietic cell stages, i.e., early myeloid progenitors that lack MPO, as well as their immediate MPO+ progeny. MPO- myeloid progenitors possess previously described granulomonocyte (GM) progenitor-associated cell-surface characteristics (CD34+CD45RA+CD13+lin-). They are specifically recruited and can be expanded in cultures of CD34+ cord blood cells in response to early-acting hematopoietic cytokines. Furthermore, cell fractions enriched in MPO- myeloid progenitors efficiently developed along Langerhans-type dendritic cell (LC) and granulomonocytic (GM) lineages, whereas progeny enriched in MPO+ cells showed diminished LC potential. In line with this, peripheral blood progenitors, known to possess LC differentiation potential, lacked MPO expression. We conclude that differential expression of MPO therefore further characterizes cells with myeloid or LC potential.
Insights
Myeloperoxidase (MPO) expression differentiates early myeloid progenitors. MPO- cells develop into Langerhans-type dendritic cells (LC) and granulomonocytic (GM) cells, while MPO+ cells show reduced LC potential.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Myeloperoxidase (MPO) is a reliable intracellular marker for myeloid lineage cells.
- MPO is present in hematopoietic progenitor cells and granulomonopoietic (GM) cells but absent in epidermal Langerhans-type dendritic cells (LC).
- This differential expression suggests MPO can subdivide myeloid progenitors functionally.
Purpose of the Study:
- To investigate the role of MPO expression in human myeloid progenitor cell differentiation.
- To determine if MPO expression can distinguish between progenitors with myeloid versus dendritic cell potential.
- To characterize distinct functional subsets of myeloid progenitors based on MPO expression.
Main Methods:
- Identification and isolation of MPO- and MPO+ myeloid progenitor populations from human hematopoietic cells.
- Culture and expansion of progenitor cells in response to hematopoietic cytokines.
- Assessment of differentiation potential towards LC and GM lineages using cell surface markers and functional assays.
Main Results:
- Two consecutive myelopoietic cell stages were identified: MPO- early myeloid progenitors and their MPO+ progeny.
- MPO- myeloid progenitors exhibited CD34+CD45RA+CD13+lin- surface characteristics.
- MPO- progenitors efficiently differentiated into LC and GM lineages, whereas MPO+ progenitors had diminished LC potential. Peripheral blood progenitors with LC potential also lacked MPO.
Conclusions:
- Differential MPO expression serves as a marker to functionally characterize human myeloid progenitor subsets.
- MPO- progenitors are precursors for both myeloid and LC lineages.
- MPO expression can predict the differentiation potential of myeloid progenitors, distinguishing those with LC potential.
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