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Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
Xanthohumol metabolites in faeces of rats
Aslieh Nookandeh1, Norbert Frank, Frank Steiner
1Institute for Pharmacognosy and Analytical Phytochemistry, University of the Saarland, 66041 Saarbrücken, Germany.
Abstract:
Xanthohumol (1), isolated from hop, was fed to rats in a dose of 1000 mg kg(-1) body weight. The faeces of the animals were collected after 24 and 48 h and analysed for metabolites of 1. Approximately 89% of the recovered flavonoid-compounds consisted of unchanged 1. Sixteen metabolites and six previously known metabolites were isolated and characterized by coupling techniques (HPLC-NMR, HPLC-MS and HPLC-DAD). Their structures were elucidated by spectroscopic methods, especially using NMR spectroscopy. Twenty metabolites had a modified chalcone structure and two metabolites were flavanone derivatives.
