Related Experiment Video

Updated: Aug 26, 2026

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
11:21

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

Amplification and one-step expression cloning of human T cell receptor genes

Ruth Moysey1, Anne-Lise Vuidepot, Jonathan M Boulter

  • 1Avidex Limited, 57-59 Milton Park, Abingdon, Oxfordshire OX14 4RX, UK.

Analytical Biochemistry
|March 9, 2004
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
08:48

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Published on: October 25, 2016

Retroviral Transduction of T-cell Receptors in Mouse T-cells
10:14

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Published on: October 22, 2010

Related Experiment Videos

Last Updated: Aug 26, 2026

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
11:21

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
08:48

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Published on: October 25, 2016

Retroviral Transduction of T-cell Receptors in Mouse T-cells
10:14

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Published on: October 22, 2010

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

Articles linked to this work by shared authors, journal, and citation graph.

Structural and biochemical studies of a moderately thermophilic exonuclease I from Methylocaldum szegediense.

PloS one·2015

Increased Peptide Contacts Govern High Affinity Binding of a Modified TCR Whilst Maintaining a Native pMHC Docking Mode.

Frontiers in immunology·2013

Production of a soluble disulfide bond-linked TCR in the cytoplasm of Escherichia coli trxB gor mutants.

Molecular biotechnology·2010

Control of HIV-1 immune escape by CD8 T cells expressing enhanced T-cell receptor.

Nature medicine·2008

T cell receptor engagement of peptide-major histocompatibility complex class I does not modify CD8 binding.

Molecular immunology·2008

Crystallization and preliminary X-ray structural studies of a Melan-A pMHC-TCR complex.

Acta crystallographica. Section F, Structural biology and crystallization communications·2007

Development and Validation of a Mechanism-Based Ba/F3-hGHR Bioassay for the Potency Assessment of Recombinant Human Growth Hormone.

Analytical biochemistry·2026

Camel Milk-Derived Extracellular Vesicles Using a Two-Step PEG Precipitation Strategy.

Analytical biochemistry·2026

Evaluation of a competitive immunochromatographic strip based on B. neotomae LPS for detecting antibodies against B. melitensis and B. abortus in cattle and goats.

Analytical biochemistry·2026

Development of immunoassay methods for ricin and application in the OPCW international proficiency test.

Analytical biochemistry·2026

A mechanistic classification framework for RT-qPCR inhibitors in wastewater-based epidemiology.

Analytical biochemistry·2026

Analytical performance comparison of Siemens Atellica CI1900 and Roche Cobas Pure integrated analyzers across spectrophotometric, ion-selective electrode and immunoassay parameters.

Analytical biochemistry·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us