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Type III secretion system genes in clinical Aeromonas isolates
M R Chacón1, L Soler, E A Groisman
1Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, 43201 Reus, Spain.
We identified Aeromonas genes ascF and ascG, key to a type III secretion system (TTSS). These TTSS genes were found in half of clinical Aeromonas isolates, particularly in Aeromonas hydrophila and Aeromonas veronii strains.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Aeromonas species are opportunistic pathogens.
- Type III secretion systems (TTSS) are virulence factors in many Gram-negative bacteria.
- The genetic basis of TTSS in Aeromonas is not fully understood.
Purpose of the Study:
- To identify and characterize genes encoding TTSS components in Aeromonas.
- To investigate the prevalence of TTSS genes in clinical Aeromonas isolates.
Main Methods:
- Gene identification using PCR and hybridization techniques.
- Analysis of 84 clinical isolates of Aeromonas.
- Species identification and prevalence assessment.
Main Results:
- Identified ascF and ascG genes encoding putative TTSS components.
- Detected TTSS gene sequences in 50% of the 84 clinical isolates.
- Found a higher prevalence of these genes in Aeromonas hydrophila and Aeromonas veronii compared to Aeromonas caviae.
Conclusions:
- Aeromonas possesses genes for a TTSS, suggesting its potential role in pathogenesis.
- The distribution of TTSS genes varies among Aeromonas species.
- Further research is needed to elucidate the function and impact of TTSS in Aeromonas infections.
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