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Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
Published on: July 16, 2012
Hepatitis C virus isolates from Argentina disclose a novel genotype 1-associated restriction pattern
María Inés Gismondi1, Lothar Heinrich Staendner, Saúl Grinstein
1Laboratorio de Virología, Hospital de Niños Ricardo Gutiérrez, C1425EFD Ciudad de Buenos Aires, Argentina. migismondi@yahoo.com.ar
Abstract:
Hepatitis C virus isolates which disclosed a novel genotype 1-associated restriction pattern by restriction fragment length polymorphism analysis were characterized. Except for a mother and child pair, the patients were unrelated. Sequence analysis showed a G-->A substitution leading to a new RsaI recognition site. Phylogenetic analysis revealed that these isolates constitute a novel genetic lineage within the main cluster of genotype 1 strains.

