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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Gene expression profiling of p53-sensitive and -resistant tumor cells using DNA microarray.

S A Maxwell1, G E Davis

  • 1Texas A&M University System Health Science Center, Department of Pathology and Laboratory Medicine, College Station, TX 77843-1114, USA. s-maxwell@tamu.edu

Apoptosis : an International Journal on Programmed Cell Death
|March 9, 2004
PubMed
Summary

Wild-type p53 overexpression triggers apoptosis in tumor cells. Researchers identified 80 novel genes involved in p53-mediated apoptosis by analyzing p53-resistant cells, offering new therapeutic targets.

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Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Wild-type p53 is a tumor suppressor that induces apoptosis.
  • Understanding p53-mediated apoptosis is crucial for cancer therapy.
  • Identifying genes involved in apoptosis resistance can reveal new therapeutic targets.

Purpose of the Study:

  • To identify novel genes involved in p53-mediated apoptosis.
  • To generate a model of p53-induced apoptosis resistance.
  • To profile gene expression differences between sensitive and resistant cells.

Main Methods:

  • Overexpression of wild-type p53 in ECV-304 tumor cells.
  • Generation of p53-resistant ECV-304 cells (DECV) via adenovirus infection.
  • DNA microarray analysis of gene expression in sensitive and resistant cells.

Main Results:

  • Identified 80 differentially expressed genes (≥2-fold change) between p53-sensitive and p53-resistant cells.
  • Many identified genes are regulated by p53 in both ECV-304 and H1299 p53-null cells.
  • Genes involved in cell cycle regulation, DNA repair, redox control, cell adhesion, apoptosis, and differentiation were identified.

Conclusions:

  • p53-resistant cells provide a valuable tool for discovering genes in p53-mediated apoptosis.
  • The study identified numerous potential p53 target genes with roles in critical cellular processes.
  • These findings may lead to new therapeutic strategies targeting p53 pathways in cancer.