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Gene expression profiling of p53-sensitive and -resistant tumor cells using DNA microarray

S A Maxwell1, G E Davis

  • 1Texas A&M University System Health Science Center, Department of Pathology and Laboratory Medicine, College Station, TX 77843-1114, USA. s-maxwell@tamu.edu

Apoptosis : an International Journal on Programmed Cell Death
|March 9, 2004
PubMed

Insights

Wild-type p53 overexpression triggers apoptosis in tumor cells. Researchers identified 80 novel genes involved in p53-mediated apoptosis by analyzing p53-resistant cells, offering new therapeutic targets.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Wild-type p53 is a tumor suppressor that induces apoptosis.
  • Understanding p53-mediated apoptosis is crucial for cancer therapy.
  • Identifying genes involved in apoptosis resistance can reveal new therapeutic targets.

Purpose of the Study:

  • To identify novel genes involved in p53-mediated apoptosis.
  • To generate a model of p53-induced apoptosis resistance.
  • To profile gene expression differences between sensitive and resistant cells.

Main Methods:

  • Overexpression of wild-type p53 in ECV-304 tumor cells.
  • Generation of p53-resistant ECV-304 cells (DECV) via adenovirus infection.
  • DNA microarray analysis of gene expression in sensitive and resistant cells.

Main Results:

  • Identified 80 differentially expressed genes (≥2-fold change) between p53-sensitive and p53-resistant cells.
  • Many identified genes are regulated by p53 in both ECV-304 and H1299 p53-null cells.
  • Genes involved in cell cycle regulation, DNA repair, redox control, cell adhesion, apoptosis, and differentiation were identified.

Conclusions:

  • p53-resistant cells provide a valuable tool for discovering genes in p53-mediated apoptosis.
  • The study identified numerous potential p53 target genes with roles in critical cellular processes.
  • These findings may lead to new therapeutic strategies targeting p53 pathways in cancer.

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