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Updated: Aug 7, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
The first green lineage cdc25 dual-specificity phosphatase
B Khadaroo1, S Robbens, C Ferraz
1Université Paris VI, Laboratoire Arago, Modèles en Biologie Cellulaire et Evolutive, Banyuls sur Mer, France.
Researchers identified the first Cdc25 protein phosphatase ortholog in the green lineage, specifically in Ostreococcus tauri. This discovery sheds light on the evolution of cell cycle regulation across different life forms.
Area of Science:
- Cell Biology
- Molecular Biology
- Evolutionary Biology
Background:
- Cdc25 protein phosphatase regulates the G(2)/M transition in metazoans and yeast.
- No Cdc25 ortholog was previously identified in plants.
- CDK-cyclin complex dephosphorylation regulates the G(2)/M transition in plants.
Purpose of the Study:
- To identify and characterize the first Cdc25 ortholog in the green lineage.
- To investigate the functional conservation of this ortholog in cell cycle regulation.
Main Methods:
- Identification of Cdc25 ortholog in Ostreococcus tauri.
- Functional rescue of yeast S. pombe cdc25-22 mutant.
- Microinjection into starfish oocytes.
- In vitro kinase assays and Western Blotting.
Main Results:
- The O. tauri Cdc25 ortholog rescues the yeast cdc25-22 mutant.
- O. tauri Cdc25 activates prophase-arrested starfish oocytes.
- Confirmed in vivo activating dephosphorylation of starfish CDK1-cyclinB by O. tauri Cdc25.
Conclusions:
- The first green lineage Cdc25 ortholog has been identified in Ostreococcus tauri.
- This ortholog is functionally conserved and can regulate M-phase entry.
- Suggests coevolution of M-phase regulatory proteins in metazoan, yeast, and green lineages.
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